National Synchrotron Radiation Research Center

About NSRRC / Staff Directory

Life Science Group

Name：	李耀昌( Yao-Chang Lee )
EXT.：	7333
E-mail：	yclee@nsrrc.org.tw
Education：	Ph.D. in Chemistry, National Tsing Hua University (2002)
Experience：	Associate research scientist Research Scientist National Synchrotron Radiation Research Center (1/ 2018~present) Assistant research scientist National Synchrotron Radiation Research Center (1/ 2004~12/2017) Adjunct Associate professor, Department of Chemistry, National Tsing Hua University (2/2020~present) Adjunct Associate professor, Department of Optics and Photonics, National Central University (8/2019~present) Adjunct Assistant professor, Department of Optics and Photonics, National Central University (8/2014~present) Adjunct Assistant professor, Department of Applied Science, National Hsinchu University of Education (8/2003~7/2012) Researcher, Industrial Technology Research Institute (8/2002~12/2003)
Research Interests：	Developing wax physisorption kinetics (WPK) for detecting aberrant glycosylation of early detection of cancer and chronic disease. Establishing artificial intelligence algorithms based on FTIR-WPK imaging data by using VB.NET in Disease Diagnosis. Developing assessment strategies of transplanted tissue by using WPK-FTIR imaging Composition analysis of paleontological samples using synchrotron-based FTIR microspectroscopy and Confocal Raman microscopy
Patent：	Y.-C. Lee and H.-W. Wang, “Real-time infrared chemical imaging spectrometer”, R.O.C. Patent, No. 203,435 (2004). Y.-C. Lee (李耀昌), “Metal-recognized apparatus and methodology by optical reflection”, R.O.C. Patent, No. I226, 435 (2005). Y.-C. Lee (李耀昌), “Multi-reflection collection probe of Fluorescence inspection”, R.O.C. Patent, No. I230, 789 (2005). Y.-C. Lee (李耀昌) and H.-W. Wang, “Real-Time infrared chemical imaging spectroscopic apparatus”, USA patent, US 6992293 B2 (2006) Y.-C. Lee (李耀昌) and P.-Y. Huang, “A novel microscope illuminator “, R.O.C. Patent, No. 09840927640(2009), Japan Patent, No. 3150912 (2009) Y.-C. Lee (李耀昌), P. Y. Huang, C.-I. Chen, Method for detecting cancer and reagents for use therein, USA patent, US-8-354,222 B2, (2013) ; Japan patent, 2009-119309, (2013) ; R.O.C. patent, No. 404931, (2013). The patent of WPK has been licensed to a third-party company in 2017. Y.-C. Lee (李耀昌), P. Y. Huang, “Method for Cancer Grading“, US 16/002,538(pending); EP18179385.2 (2018)(pending)
Selected Publications：	C.-J. Liu, C.-H. Wang, C.-C Chien, T.-Y. Yang, S.-T Chen, W.-H. Leng, C.-F. Lee, K.-H. Lee, Y. Hwu, Y.-C. Lee, C.-L. Cheng, C.-S. Yang, Y.-J. Chen, J.-H. Je and G. Margaritondo, “Enhanced x-ray irradiation-induced cancer cell damage by gold nanoparticles treated by a new synthesis method of polyethylene glycol modification”, Nanotechnology, 19, 295104(2008) Y.-C. Lin, H.-L. Chou, M.-C. Tsai, B.-J. Hwang, L. S. Sarma, Y.-C. Lee and C.-I. Chen, “Combined Experimental and Theoretical Investigation of Nanosized Effects of Pt Catalyst on Their Underlying Methanol Electro-Oxidation Activity”, Phys. Chem. C, 113 (21), 9197(2009) C.-J. Liu, T.-Y. Yang, C.-H.Wang, C.-C. Chien, S.-T. Chen, C.-L.Wang, W.-H. Leng, Y. Hwu, H.-M. Line, Y.-C. Lee, C.-L. Cheng, J. H. Je , G. Margaritondo, “Enhanced photocatalysis, colloidal stability and cytotoxicity of synchrotron X-ray synthesized Au/TiO2 nanoparticles”, Materials Chemistry and Physics, 117 74(2009) Y.-C. Lee, C.-I. Chen, P.-Y. Huang, and C.-Y. Liu, “A study of Fourier-Transform infrared Microspectroscopy under Top-Up injection Operation at NSRRC”, Instruments today.170, 7-15 (2009) Y.-C. Lee, P.-Y. Huang , Y.-C. Chang, and C.-I. Chen, “An infrared kinetic study of wax physisorption for oral cavity cancer detection using synchrotron-based infrared microspectrscopy”, Instruments today.170, 47-60 (2009) J. B. Aitken, E. A. Carter, H. Eastgate, M. J. Hackett, H. H. Harris, A. Levina, Y.-C. Lee, C.-I Chen, B. Laid, S. Vogt, P. A. Lay, “Biomedical application soft X-ray absorption and vibrational spectroscopic microscopies in obtaining structural information from complex systems”, Radiation Physics and Chemistry 79, 176 (2010) C.-Y. Chung, Y.-Y. Lin, K.-Y. Wu, W.-Y. Tai, S.-W. Chu, Y.-C. Lee, Y. Hwu, Y.-Y. Lee, “Coherent anti-Stokes Raman scattering microscopy using a single-pass picosecond supercontinuum-seeded optical parametricamplifier”, Optics Express, 18(6), 6116(2010) E. A. Carter, B. S. Rayner, A. I. McLeod, L. E. Wu, C. P. Marshall, A. Levina, J. B. Aitken, P. K. Witting, B. Lai, Z. Cai, S. Vogt, Y.-C. Lee, C.-I. Chen, M. J. Tobin, H. H. Harris and P. A. Lay “Silicon nitride as a versatile growth substrate for microspectroscopic imaging and mapping of individual cells ” Mol. BioSyst., 6, 1316 – 1322(2010) (IF: 4.23) C.-W. Huang, Y.-C. Lee, and Y.-P. Lee “Diminished cage effect in solid p-H2: Infrared spectra of ClSCS, ClCS, and ClSC in an irradiated p-H2 matrix containing Cl2 and CS2” J. Chem. Phys. 132, 164303 (2010) (IF:3.093) A.H.M. S. Reza, J.-S. Jean, M.-K. Lee, C.-C. Liu, J. Bundschuh, H.-J. Yang, J.-F. Lee, Y.-C. Lee,“ Implications of organic matter on arsenic mobilization into groundwater: Evidence from northwestern (Chapai-Nawabganj), central (Manikganj) and southeastern (Chadpur) Bangladesh”, Water Research, 44(19), 5556–74 (2010), (IF:4.35) H.-C. Lu, W.-T. Whang, and B.-M. Cheng, "Effect of Alkyl Position of Pyrrole on Structures and Properties of Conjugated Polysquaraines ", Synthetic Metals 160, 1002-1007 (2010). (IF:1.91) H.-C. Lu, W.-T. Whang and B.-M. Cheng "Switchable Structural Modification Accompanying Altered Optical Properties of a Zwitterionic Polysquaraine", Chem. Phys. Lett. 500, 267-271 (2010) (IF:2.291) A. H. M. S. Reza, J.-S. Jean, H.-J. Yang, M.-K. Lee, H.-F. Hsu, C.-C. Liu, Y.-C. Lee, J. Bundschuh, K.-H. Lin, C.-Y. Lee, “A comparative study on arsenic and humic substances in alluvial aquifers of Bengal delta plain (NW Bangladesh), Chianan plain (SW Taiwan) and Lanyang plain (NE Taiwan): implication of arsenic mobilization mechanisms” Environ Geochem Health, 33(3), 235 –58 (2011) (IF:1.622) S. Kar, J. P. Maity, J.-S. Jean, C.-C. Liu, B. Nath, Y.-C. Lee, J. Bundschuh, C.-Y. Chen and Z. Li, “Role of organic matter and humic substances in the binding and mobility of arsenic in a Gangetic aquifer”, Journal of Environmental Science and Health, Part A, 46, 1231–1238(2011). (IF:1.36) J. M. Chen, S. C. Haw, J. M. Lee, T. L. Chou, S. A. Chen, K. T. Lu, Y. C. Liang, Y. C. Lee, N. Hiraoka, H. Ishii, K. D. Tsuei, Eugene Huang, and T. J. Yang, Pressure dependence of the electronic structure and spin state in Fe1.01Se superconductors probed by x-ray absorption and x-ray emission spectroscopy, PHYSICAL REVIEW B, 84, 125117(2011). (IF:3.28) Y.-H. Chen, J.-S. Jean?, C.-C. Liu, Y.-C. Lee, Kinetics and mechanism of arsenate removal by nanosized iron oxide-coated perlite M.G. Mostafa, Journal of Hazardous Materials, 187, 89–95 (2011). (IF:3.997) F. Taufany, C.-J. Pan, J. Rick, H.-L. Chou, M.-C. Tsai, B.-J. Hwang, D.-G. Liu, J.-F. Lee, M.-T. Tang, Y.-C. Lee, and C.-I. Chen, Kinetically Controlled Autocatalytic Chemical Process for Bulk Production of Bimetallic Core_Shell Structured Nanoparticles, ACS nano, 5(12), 9370–9381(2011). (IF:9.855) S.-E. Peng, C.-S. Chen, Y.-F. Song, H.-T. Huang, P.-L. Jiang, W.-N. U. Chen, L.-S. Fang and Y.-C. Lee, “Assessment of metabolic modulation in free-living versus endosymbiotic Symbiodinium using synchrotron radiation-based infrared microspectroscopy” Biol. Lett. 8(3), 434-437 (2012) (IF:3.651) A. H. M. S. Reza, J.-S. Jean, M.-K. Lee, T. R. Kulp, H.-F. Hsu,C.-C. Liu, Y.-C. Lee, “The binding nature of humic substances with arsenic in alluvial aquifers of Chianan Plain, southwestern Taiwan” Journal of Geochemical Exploration, 114 98–108 (2012).(IF:1.312) C.-C. Liu, S. Kara, J.-S. Jean, C.-H. Wang, Y.-C. Lee, O. Sracek, Z. Li, J. Bundschuh, H.-J. Yang, C.-Y. Chen, ”Linking geochemical processes in mud volcanoes with arsenic mobilization driven by organic matter”, Journal of Hazardous Materials, 262, 980– 988 (2013). (IF:4.553) L.-F. Chiu, P.-Y. Huang, W.-F. Chiang, T.-Y. Wong, S.-H. Lin, Y.-C. Lee, D.-B. Shieh, “Oral cancer diagnostics based on infrared spectral markers and wax physisorption kinetics”, Anal. Bioanal. Chem. 405(6), 1995-2007 (2013) (IF:3.841) P.-Q Yu, K. Theodoridou, H. Xin, P.-Y. Huang, Y.-C. Lee, B. R. Wood, J. Agric. Food Chem. 61, 7234(2013) (IF:2.906) C.-K. Chen, M.-H. Chang, H.-T. Wu, Y.-C. Lee (李耀昌), T.-J. Yen, “Enhanced vibrational spectroscopy, intracellular refractive indexing for label-free biosensing and bioimaging by multiband plasmonic-antenna array”, Biosens. Bioelectron., 60, 343 (2014). (IF: 6.451) (citation:9) M. M. H. Hsu, P.-Y. Huang, Y.-C. Lee (李耀昌), Y.-C. Fang, M. W. Y. Chan, and C.-I. Lee, “FT-IR Microspectrometry Reveals the Variation of Membrane Polarizability due to Epigenomic Effect on Epithelial Ovarian Cancer”, Int. J. Mol. Sci., 15(10): 17963–17973, (2014). (IF: 2.339) (citation:4) K. S. Brink, R. R. Reisz, A. R. H. LeBlanc, R.-S. Chang, Y.-C. Lee (李耀昌), C.-C. Chiang, T. Huang, and D. C. Evans, “Developmental and evolutionary novelty in the serrated teeth of theropod dinosaurs”, Scientific Reports 5, Article number: 12338, DOI: 10.1038/srep12338 (2015). (IF: 5.578) (citation:11) C.-H. Lee, C.-Y. Hsu, P.-Y. Huang, C.-I. Chen, Y.-C. Lee* (李耀昌), and H.-S. Yu, “Arsenite Regulates Prolongation of Glycan Residues of Membrane Glycoprotein: A Pivotal Study via Wax Physisorption Kinetics and FTIR Imaging”, Int. J. Mol. Sci., 17, 427 (2016). (IF: 3.257) (citation:1) A. Kunz, B. A. Walther, L. Löwemark, Y.-C. Lee (李耀昌), “Distribution and quantity of microplastic on sandy beaches along the northern coast of Taiwan”, Marine Pollution Bulletin, 111, 126 (2016). (IF: 2.68) (citation:2) M.-H. Lin, S. Hy, C.-Y. Chen, J.-H. Cheng, J. Rick, N.-W. Pu, W.-N. Su, Y.-C. Lee (李耀昌) and B.-J. Hwang, “Resilient Yolk-Shell Silicon-Reduced Graphene Oxide/Amorphous Carbon Anode Material Achieved by a Synergistic Dual-Coating Process for Lithium-Ion Batteries”, ChemElectroChem 10.1002/celc.201600254 (2016). (IF: 3.506) (citation:4) C.-H. Lee, C.-Y. Hsu, P.-Y. Huang, C.-I. Chen, Y.-C. Lee (李耀昌), and H.-S. Yu, “Arsenite Regulates Prolongation of Glycan Residues of Membrane Glycoprotein: A Pivotal Study via Wax Physisorption Kinetics and FTIR Imaging”, Int. J. Mol. Sci., 17, 427 (2016). (IF: 3.257) (citation:1) W.-T. Chuang, Y.-M. Hsu, E.-L. Lin, I-M. Lin, Y.-S. Sun, Y.-W. Chiang, C.-J. Su, Y.-C. Lee (李耀昌), U-S. Jeng, "Live Templates of a Supramolecular Block Copolymer for the Synthesis of Ordered Nanostructured TiO2 Films via Guest Exchange", ACS Applied Materials & Interfaces, DOI: 10.1021/acsami.6b12216 (2016). (IF: 7.145) (citation:0) Y.-C. Lee (李耀昌), C.-C. Chiang, P.-Y. Huang, C.-Y. Chung, T. D. Huang, C.-C. Wang, C.-I. Chen, R.-S. Chang, C.-H. Liao, R. R. Reisz, “Evidence of preserved collagen in an Early Jurassic sauropodomorph dinosaur revealed by synchrotron FTIR microspectroscopy”, Nature Communications, 8, 14220, DOI: 10.1038/ncomms14220 (2017). (IF: 12.124) (citation:2) C.-W. Chen, W.-J. Syu, T.-C. Huang, Y.-C. Lee (李耀昌), J.-K. Hsiao, K.-Y. Huang, H.-P. Yu, M.-Y. Liao and P.-S. Lai,” Encapsulation of Au/Fe3O4 nanoparticles into a polymer nanoarchitecture with combined near infrared-triggered chemo-photothermal therapy based on intracellular secondary protein understanding”, J. Mater. Chem. B, 5, 5774 (2017) (IF: 4.543) Y.-H. Hsieh, J.-H. Zhang, W.-C. Chuang, K.-H. Yu, X.-B. Huang, Y.-C. Lee(李耀昌), and C.-I. Lee*, “An in Vitro Study on the Effect of Combined Treatment with Photodynamic and Chemical Therapies on Candida Albicans”, Int. J. Mol. Sci. 19 , 337 (2018) (IF:3.226) A. Priprem, Y.-C. Lee (李耀昌), W. Limphirat, S. Tiyaworanant, K. Saodaeng, J. Chotitumnavee, N. Kowtragoon, “Eucalyptus ash alters secondary protein conformation of human grey hair and facilitates anthocyanin dyeing” PLoS ONE 13(7): e0199696 (2018) https://doi.org/10.1371/journal.pone.0199696 (IF: 2.806)