About NSRRC / Staff Directory

Life Science Group

Name: 李耀昌( Yao-Chang Lee )
EXT.: 7333
E-mail: yclee@nsrrc.org.tw
  • Ph.D. in Chemistry, National Tsing Hua University (2002)
  • Associate research scientist Research Scientist National Synchrotron Radiation Research Center (1/ 2018~present)
  • Assistant research scientist National Synchrotron Radiation Research Center (1/ 2004~12/2017)
  • Adjunct Associate professor, Department of Chemistry, National Tsing Hua University (2/2020~present)
  • Adjunct Associate professor, Department of Optics and Photonics, National Central University (8/2019~present)
  • Adjunct Assistant professor, Department of Optics and Photonics, National Central University (8/2014~present)
  • Adjunct Assistant professor, Department of Applied Science, National Hsinchu University of Education (8/2003~7/2012)
  • Researcher, Industrial Technology Research Institute (8/2002~12/2003)
Research Interests:
  • Developing wax physisorption kinetics (WPK) for detecting aberrant glycosylation of early detection of cancer and chronic disease.
  • Establishing artificial intelligence algorithms based on FTIR-WPK imaging data by using VB.NET  in Disease Diagnosis.
  • Developing assessment strategies of transplanted tissue by using WPK-FTIR imaging
  • Composition analysis of paleontological samples using synchrotron-based FTIR microspectroscopy and Confocal Raman microscopy
  •  Y.-C. Lee and H.-W. Wang, “Real-time infrared chemical imaging spectrometer”, R.O.C. Patent, No. 203,435 (2004).
  • Y.-C. Lee (李耀昌), “Metal-recognized apparatus and methodology by optical reflection”, R.O.C. Patent, No. I226, 435 (2005).
  • Y.-C. Lee (李耀昌), “Multi-reflection collection probe of Fluorescence inspection”, R.O.C. Patent, No. I230, 789 (2005).
  • Y.-C. Lee (李耀昌) and H.-W. Wang, “Real-Time infrared chemical imaging spectroscopic apparatus”, USA patent, US 6992293 B2 (2006)
  • Y.-C. Lee (李耀昌) and P.-Y. Huang, “A novel microscope illuminator “, R.O.C. Patent, No. 09840927640(2009), Japan Patent, No. 3150912 (2009)
  • Y.-C. Lee (李耀昌), P. Y. Huang, C.-I. Chen, Method for detecting cancer and reagents for use therein, USA patent, US-8-354,222 B2, (2013) ; Japan patent, 2009-119309, (2013) ; R.O.C. patent, No. 404931, (2013). The patent of WPK has been licensed to a third-party company in 2017.
  • Y.-C. Lee (李耀昌), P. Y. Huang, “Method for Cancer Grading“, US 16/002,538(pending); EP18179385.2 (2018)(pending)
Selected Publications:
  • C.-J. Liu, C.-H. Wang, C.-C Chien, T.-Y. Yang, S.-T Chen, W.-H. Leng, C.-F. Lee, K.-H. Lee, Y. Hwu, Y.-C. Lee, C.-L. Cheng, C.-S. Yang, Y.-J. Chen, J.-H. Je and G. Margaritondo, “Enhanced x-ray irradiation-induced cancer cell damage by gold nanoparticles treated by a new synthesis method of polyethylene glycol modification”, Nanotechnology, 19, 295104(2008)
  • Y.-C. Lin, H.-L. Chou, M.-C. Tsai, B.-J. Hwang, L. S. Sarma, Y.-C. Lee and C.-I. Chen, “Combined Experimental and Theoretical Investigation of Nanosized Effects of Pt Catalyst on Their Underlying Methanol Electro-Oxidation Activity”, Phys. Chem. C, 113 (21), 9197(2009)
  • C.-J. Liu, T.-Y. Yang, C.-H.Wang, C.-C. Chien, S.-T. Chen, C.-L.Wang, W.-H. Leng, Y. Hwu*, H.-M. Line, Y.-C. Lee, C.-L. Cheng, J. H. Je , G. Margaritondo, “Enhanced photocatalysis, colloidal stability and cytotoxicity of synchrotron X-ray synthesized Au/TiO2 nanoparticles”, Materials Chemistry and Physics, 117 74(2009)
  • Y.-C. Lee*, C.-I. Chen, P.-Y. Huang, and C.-Y. Liu, “A study of Fourier-Transform infrared Microspectroscopy under Top-Up injection Operation at NSRRC”, Instruments today.170, 7-15 (2009)
  • Y.-C. Lee*, P.-Y. Huang , Y.-C. Chang, and C.-I. Chen, “An infrared kinetic study of wax physisorption for oral cavity cancer detection using synchrotron-based infrared microspectrscopy”, Instruments today.170, 47-60 (2009)
  • J. B. Aitken, E. A. Carter, H. Eastgate, M. J. Hackett, H. H. Harris, A. Levina, Y.-C. Lee, C.-I Chen, B. Laid, S. Vogt, P. A. Lay*, “Biomedical application soft X-ray absorption and vibrational spectroscopic microscopies in obtaining structural information from complex systems”, Radiation Physics and Chemistry 79, 176 (2010)
  • C.-Y. Chung, Y.-Y. Lin, K.-Y. Wu, W.-Y. Tai, S.-W. Chu, Y.-C. Lee, Y. Hwu, Y.-Y. Lee*, “Coherent anti-Stokes Raman scattering microscopy using a single-pass picosecond supercontinuum-seeded optical parametricamplifier”, Optics Express, 18(6), 6116(2010)
  • E. A. Carter, B. S. Rayner, A. I. McLeod, L. E. Wu, C. P. Marshall, A. Levina, J. B. Aitken, P. K. Witting, B. Lai, Z. Cai, S. Vogt, Y.-C. Lee, C.-I. Chen, M. J. Tobin, H. H. Harris and P. A. Lay “Silicon nitride as a versatile growth substrate for microspectroscopic imaging and mapping of individual cells ” Mol. BioSyst., 6, 1316 – 1322(2010) (IF: 4.23)
  • C.-W. Huang, Y.-C. Lee, and Y.-P. Lee* “Diminished cage effect in solid p-H2: Infrared spectra of ClSCS, ClCS, and ClSC in an irradiated p-H2 matrix containing Cl2 and CS2” J. Chem. Phys. 132, 164303 (2010) (IF:3.093)
  • A.H.M. S. Reza, J.-S. Jean*, M.-K. Lee, C.-C. Liu, J. Bundschuh, H.-J. Yang, J.-F. Lee, Y.-C. Lee,“ Implications of organic matter on arsenic mobilization into groundwater: Evidence from northwestern (Chapai-Nawabganj), central (Manikganj) and southeastern (Chadpur) Bangladesh”, Water Research, 44(19), 5556–74 (2010), (IF:4.35)
  • H.-C. Lu, W.-T. Whang, and B.-M. Cheng, "Effect of Alkyl Position of Pyrrole on Structures and Properties of Conjugated Polysquaraines ", Synthetic Metals 160, 1002-1007 (2010). (IF:1.91)
  • H.-C. Lu, W.-T. Whang and B.-M. Cheng "Switchable Structural Modification Accompanying Altered Optical Properties of a Zwitterionic Polysquaraine", Chem. Phys. Lett. 500, 267-271 (2010) (IF:2.291)
  • A. H. M. S. Reza, J.-S. Jean, H.-J. Yang, M.-K. Lee, H.-F. Hsu, C.-C. Liu, Y.-C. Lee, J. Bundschuh, K.-H. Lin, C.-Y. Lee, “A comparative study on arsenic and humic substances in alluvial aquifers of Bengal delta plain (NW Bangladesh), Chianan plain (SW Taiwan) and Lanyang plain (NE Taiwan): implication of arsenic mobilization mechanisms” Environ Geochem Health, 33(3), 235 –58 (2011) (IF:1.622)
  • S. Kar, J. P. Maity, J.-S. Jean, C.-C. Liu, B. Nath, Y.-C. Lee, J. Bundschuh, C.-Y. Chen and Z. Li, “Role of organic matter and humic substances in the binding and mobility of arsenic in a Gangetic aquifer”, Journal of Environmental Science and Health, Part A, 46, 1231–1238(2011). (IF:1.36)
  • J. M. Chen*, S. C. Haw, J. M. Lee, T. L. Chou, S. A. Chen, K. T. Lu, Y. C. Liang, Y. C. Lee, N. Hiraoka, H. Ishii, K. D. Tsuei, Eugene Huang, and T. J. Yang, Pressure dependence of the electronic structure and spin state in Fe1.01Se superconductors probed by x-ray absorption and x-ray emission spectroscopy, PHYSICAL REVIEW B, 84, 125117(2011). (IF:3.28)
  • Y.-H. Chen, J.-S. Jean?, C.-C. Liu, Y.-C. Lee, Kinetics and mechanism of arsenate removal by nanosized iron oxide-coated perlite M.G. Mostafa, Journal of Hazardous Materials, 187, 89–95 (2011). (IF:3.997)
  • F. Taufany, C.-J. Pan, J. Rick, H.-L. Chou, M.-C. Tsai, B.-J. Hwang*, D.-G. Liu, J.-F. Lee, M.-T. Tang, Y.-C. Lee, and C.-I. Chen, Kinetically Controlled Autocatalytic Chemical Process for Bulk Production of Bimetallic Core_Shell Structured Nanoparticles, ACS nano, 5(12), 9370–9381(2011). (IF:9.855)
  • S.-E. Peng*, C.-S. Chen, Y.-F. Song, H.-T. Huang, P.-L. Jiang, W.-N. U. Chen, L.-S. Fang and Y.-C. Lee*, “Assessment of metabolic modulation in free-living versus endosymbiotic Symbiodinium using synchrotron radiation-based infrared microspectroscopy” Biol. Lett. 8(3), 434-437 (2012) (IF:3.651)
  • A. H. M. S. Reza, J.-S. Jean, M.-K. Lee, T. R. Kulp, H.-F. Hsu,C.-C. Liu, Y.-C. Lee, “The binding nature of humic substances with arsenic in alluvial aquifers of Chianan Plain, southwestern Taiwan” Journal of Geochemical Exploration, 114 98–108 (2012).(IF:1.312)
  • C.-C. Liu, S. Kara, J.-S. Jean, C.-H. Wang, Y.-C. Lee, O. Sracek, Z. Li, J. Bundschuh, H.-J. Yang, C.-Y. Chen, ”Linking geochemical processes in mud volcanoes with arsenic mobilization driven by organic matter”, Journal of Hazardous Materials, 262, 980– 988 (2013). (IF:4.553)
  • L.-F. Chiu, P.-Y. Huang, W.-F. Chiang, T.-Y. Wong, S.-H. Lin, Y.-C. Lee*, D.-B. Shieh*, “Oral cancer diagnostics based on infrared spectral markers and wax physisorption kinetics”, Anal. Bioanal. Chem. 405(6), 1995-2007 (2013) (IF:3.841)
  • P.-Q Yu, K. Theodoridou, H. Xin, P.-Y. Huang, Y.-C. Lee, B. R. Wood, J. Agric. Food Chem. 61, 7234(2013) (IF:2.906)
  • C.-K. Chen, M.-H. Chang, H.-T. Wu, Y.-C. Lee (李耀昌), T.-J. Yen, “Enhanced vibrational spectroscopy, intracellular refractive indexing for label-free biosensing and bioimaging by multiband plasmonic-antenna array”, Biosens. Bioelectron., 60, 343 (2014). (IF: 6.451) (citation:9)
  • M. M. H. Hsu, P.-Y. Huang, Y.-C. Lee (李耀昌), Y.-C. Fang, M. W. Y. Chan, and C.-I. Lee*, “FT-IR Microspectrometry Reveals the Variation of Membrane Polarizability due to Epigenomic Effect on Epithelial Ovarian Cancer”, Int. J. Mol. Sci., 15(10): 17963–17973, (2014). (IF: 2.339) (citation:4)
  • K. S. Brink*, R. R. Reisz*, A. R. H. LeBlanc, R.-S. Chang, Y.-C. Lee (李耀昌), C.-C. Chiang, T. Huang, and D. C. Evans, “Developmental and evolutionary novelty in the serrated teeth of theropod dinosaurs”, Scientific Reports 5, Article number: 12338, DOI: 10.1038/srep12338 (2015). (IF: 5.578) (citation:11)
  • C.-H. Lee, C.-Y. Hsu, P.-Y. Huang, C.-I. Chen, Y.-C. Lee* (李耀昌), and H.-S. Yu*, “Arsenite Regulates Prolongation of Glycan Residues of Membrane Glycoprotein: A Pivotal Study via Wax Physisorption Kinetics and FTIR Imaging”, Int. J. Mol. Sci., 17, 427 (2016). (IF: 3.257) (citation:1)
  • A. Kunz*, B. A. Walther, L. Löwemark, Y.-C. Lee (李耀昌), “Distribution and quantity of microplastic on sandy beaches along the northern coast of Taiwan”, Marine Pollution Bulletin, 111, 126 (2016). (IF: 2.68) (citation:2)
  • M.-H. Lin, S. Hy, C.-Y. Chen, J.-H. Cheng, J. Rick, N.-W. Pu, W.-N. Su, Y.-C. Lee (李耀昌) and B.-J. Hwang*, “Resilient Yolk-Shell Silicon-Reduced Graphene Oxide/Amorphous Carbon Anode Material Achieved by a Synergistic Dual-Coating Process for Lithium-Ion Batteries”, ChemElectroChem 10.1002/celc.201600254 (2016). (IF: 3.506) (citation:4)
  • C.-H. Lee, C.-Y. Hsu, P.-Y. Huang, C.-I. Chen, Y.-C. Lee* (李耀昌), and H.-S. Yu*, “Arsenite Regulates Prolongation of Glycan Residues of Membrane Glycoprotein: A Pivotal Study via Wax Physisorption Kinetics and FTIR Imaging”, Int. J. Mol. Sci., 17, 427 (2016). (IF: 3.257) (citation:1)
  • W.-T. Chuang, Y.-M. Hsu, E.-L. Lin, I-M. Lin, Y.-S. Sun, Y.-W. Chiang, C.-J. Su, Y.-C. Lee (李耀昌), U-S. Jeng, "Live Templates of a Supramolecular Block Copolymer for the Synthesis of Ordered Nanostructured TiO2 Films via Guest Exchange", ACS Applied Materials & Interfaces, DOI: 10.1021/acsami.6b12216 (2016). (IF: 7.145) (citation:0)
  • Y.-C. Lee* (李耀昌), C.-C. Chiang, P.-Y. Huang, C.-Y. Chung, T. D. Huang, C.-C. Wang, C.-I. Chen, R.-S. Chang, C.-H. Liao, R. R. Reisz*, “Evidence of preserved collagen in an Early Jurassic sauropodomorph dinosaur revealed by synchrotron FTIR microspectroscopy”, Nature Communications, 8, 14220, DOI: 10.1038/ncomms14220 (2017). (IF: 12.124) (citation:2)
  • C.-W. Chen, W.-J. Syu, T.-C. Huang, Y.-C. Lee (李耀昌), J.-K. Hsiao, K.-Y. Huang, H.-P. Yu, M.-Y. Liao and P.-S. Lai*,” Encapsulation of Au/Fe3O4 nanoparticles into a polymer nanoarchitecture with combined near infrared-triggered chemo-photothermal therapy based on intracellular secondary protein understanding”, J. Mater. Chem. B, 5, 5774 (2017) (IF: 4.543)
  • Y.-H. Hsieh, J.-H. Zhang, W.-C. Chuang, K.-H. Yu, X.-B. Huang, Y.-C. Lee(李耀昌), and C.-I. Lee*, “An in Vitro Study on the Effect of Combined Treatment with Photodynamic and Chemical Therapies on Candida Albicans”, Int. J. Mol. Sci.  19 , 337 (2018) (IF:3.226)
  • A. Priprem, Y.-C. Lee (李耀昌), W. Limphirat, S. Tiyaworanant, K. Saodaeng, J. Chotitumnavee, N. Kowtragoon, “Eucalyptus ash alters secondary protein conformation of human grey hair and facilitates anthocyanin dyeing” PLoS ONE 13(7): e0199696 (2018) https://doi.org/10.1371/journal.pone.0199696 (IF: 2.806)