中心簡介 / 同仁通訊錄

X光影像小組


姓名： 陳建樺( Jian-Hua Chen )
分機： 7309
電子郵件： chen.jh@nsrrc.org.tw
學歷：
  • 德國馬克斯普郎克研究所/哥廷根大學 物理學博士 (2013)
  • 國立陽明交通大學 生醫光電研究所 碩士 (2004)
  • 國立中央大學 電機工程系 學士 (2002)
經歷：
  • 國家同步輻射研究中心 副研究員 (2025.01-present) 
  • 加州大學舊金山分校/勞倫斯柏克萊國家實驗室  Staff Scientist (2015-2024) 
  • 加州大學舊金山分校/勞倫斯柏克萊國家實驗室  Postdoc (2014-2015)   
負責實驗站：
  • TPS 24A Soft X-ray Tomography
研究領域：
  • 三維細胞影像 
  • 細胞螢光影像與動態分析
  • 利用超高解析度顯微技術探討疾病機制
代表作：
  • My Google Scholar 
  • Chatzimpinou, A., Diehl, A., Harhoff, A. T., Driller, K., Vanslembrouck, B., Chen, J.-H., Kairišs, K., Loconte, V., Gros, M. A. L., Larabell, C., Turgay, K., Oschkinat, H. & Weinhardt, V. Soft X-ray tomography reveals variations in B. subtilis biofilm structure upon tasA deletion. npj Biofilms Microbiomes 11, 23 (2025).
  • Hueschen, C. L., Segev-Zarko, L., Chen, J.-H., LeGros, M. A., Larabell, C. A., Boothroyd, J. C., Phillips, R. & Dunn, A. R. Emergent actin flows explain distinct modes of gliding motility. Nat. Phys. 20, 1989–1996 (2024).
  • Chen, J.-H., Mirvis, M., Ekman, A., Vanslembrouck, B., Gros, M. L., Larabell, C. & Marshall, W. F. Automated segmentation of soft X-ray tomography: native cellular structure with sub-micron resolution at high throughput for whole-cell quantitative imaging in yeast. bioRxiv 2024–10 (2024).
  • Coale, T. H., Loconte, V., Turk-Kubo, K. A., Vanslembrouck, B., Mak, W. K. E., Cheung, S., Ekman, A., Chen, J.-H., Hagino, K. & Takano, Y. Nitrogen-fixing organelle in a marine alga. Science 384, 217–222 (2024).
  • Sontag, E. M., Morales-Polanco, F., Chen, J.-H., McDermott, G., Dolan, P. T., Gestaut, D., Gros, M. A. L., Larabell, C. & Frydman, J. Nuclear and cytoplasmic spatial protein quality control is coordinated by nuclear–vacuolar junctions and perinuclear ESCRT. Nature Cell Biology 25, 699–713 (2023).
  • Ekman, A., Chen, J.-H., Vanslembrouck, B., Loconte, V., Larabell, C. A., Gros, M. A. L. & Weinhardt, V. Extending imaging volume in soft X‐Ray tomography. Advanced Photonics Research 4, 2200142 (2023).
  • Eng, T., Banerjee, D., Menasalvas, J., Chen, Y., Gin, J., Choudhary, H., Baidoo, E., Chen, J. H., Ekman, A. & Kakumanu, R. Maximizing microbial bioproduction from sustainable carbon sources using iterative systems engineering. Cell Reports 42, (2023).
  • Loconte, V., Chen, J.-H., Vanslembrouck, B., Ekman, A. A., McDermott, G., Gros, M. A. L. & Larabell, C. A. Soft X‐ray tomograms provide a structural basis for whole‐cell modeling. The FASEB Journal 37, e22681 (2023).
  • Vanslembrouck, B., Chen, J.-H, Larabell, C. & Hengel, J. van. Microscopic visualization of cell-cell adhesion complexes at micro and nanoscale. Frontiers in cell and developmental biology 10, 819534 (2022)
  • Chen, J.-H., Vanslembrouck, B., Ekman, A., Aho, V., Larabell, C. A., Gros, M. A. L., Vihinen-Ranta, M. & Weinhardt, V. Soft X-ray tomography reveals HSV-1-induced remodeling of human B cells. Viruses 14, 2651 (2022).
  • Chen, J.-H., Vanslembrouck, B., Loconte, V., Ekman, A., Cortese, M., Bartenschlager, R., McDermott, G., Larabell, C. A., Gros, M. A. L. & Weinhardt, V. A protocol for full-rotation soft X-ray tomography of single cells. Star Protoc 3, 101176 (2022).
  • Loconte, V., Singla, J., Li, A., Chen, J.-H., Ekman, A., McDermott, G., Sali, A., Gros, M. L., White, K. L. & Larabell, C. A. Soft X-ray tomography to map and quantify organelle interactions at the mesoscale. Structure 30, 510–521 (2022).
  • (co-first) Loconte, V*., Chen, J.-H.*, Cortese, M., Ekman, A., Gros, M. A. L., Larabell, C., Bartenschlager, R. & Weinhardt, V. Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells. Cell reports methods 1, (2021).
  • Remesh, S. G., Verma, S. C., Chen, J.-H., Ekman, A. A., Larabell, C. A., Adhya, S. & Hammel, M. Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling. Nature communications 11, 2905 (2020).
  • Weinhardt, V., Chen, J.-H., Ekman, A. A., Guo, J., Remesh, S. G., Hammel, M., McDermott, G., Chao, W., Oh, S. & Gros, M. A. L. Switchable resolution in soft x-ray tomography of single cells. PLoS One 15, e0227601 (2020).
  • Polo, C. C., Fonseca-Alaniz, M. H., Chen, J.-H., Ekman, A., McDermott, G., Meneau, F., Krieger, J. E. & Miyakawa, A. A. Three-dimensional imaging of mitochondrial cristae complexity using cryo-soft X-ray tomography. Scientific reports 10, 21045 (2020).
  • Ekman, A., Chen, J.-H., Weinhardt, V., Do, M., McDermott, G., Gros, M. A. L. & Larabell, C. A. Putting molecules in the picture: using correlated light microscopy and soft X-ray tomography to study cells. Synchrotron Light Sources and Free-Electron Lasers: Accelerator Physics, Instrumentation and Science Applications 1613–1644 (2020).
  • White, K. L., Singla, J., Loconte, V., Chen, J.-H., Ekman, A., Sun, L., Zhang, X., Francis, J. P., Li, A. & Lin, W. Visualizing subcellular rearrangements in intact β cells using soft x-ray tomography. Science advances 6, eabc8262 (2020).
  • Weinhardt, V., Chen, J.-H., Ekman, A., McDermott, G., Gros, M. A. L. & Larabell, C. Imaging cell morphology and physiology using X-rays. Biochemical Society Transactions 47, 489–508 (2019).
  • Walters, A. D., Amoateng, K., Wang, R., Chen, J.-H., McDermott, G., Larabell, C. A., Gadal, O. & Cohen-Fix, O. Nuclear envelope expansion in budding yeast is independent of cell growth and does not determine nuclear volume. Molecular biology of the cell 30, 131–145 (2019).
  • Roth, M. S., Gallaher, S. D., Westcott, D. J., Iwai, M., Louie, K. B., Mueller, M., Walter, A., Foflonker, F., Bowen, B. P. & Ataii, N. N., Song, J., Chen, J.-H., Regulation of oxygenic photosynthesis during trophic transitions in the green alga Chromochloris zofingiensis. The Plant Cell 31, 579–601 (2019).
  • Aho, V., Mäntylä, E., Ekman, A., Hakanen, S., Mattola, S., Chen, J.-H., Weinhardt, V., Ruokolainen, V., Sodeik, B. & Larabell, C. Quantitative microscopy reveals stepwise alteration of chromatin structure during herpesvirus infection. Viruses 11, 935 (2019).
  • Ekman, A., Weinhardt, V., Chen, J.-H., McDermott, G., Gros, M. A. L. & Larabell, C. PSF correction in soft X-ray tomography. Journal of structural biology 204, 9–18 (2018).
  • Mehta, A. P., Supekova, L., Chen, J.-H., Pestonjamasp, K., Webster, P., Ko, Y., Henderson, S. C., McDermott, G., Supek, F. & Schultz, P. G. Engineering yeast endosymbionts as a step toward the evolution of mitochondria. Proceedings of the National Academy of Sciences 115, 11796–11801 (2018).
  • Ekman, A. A., Plautz, T. E., Chen, J.-H., McDermott, G., Gros, M. A. L. & Larabell, C. A. in Handbook of X-ray Imaging 817–852 (CRC Press, 2017).
  • Ekman, A. A., Chen, J.-H., Guo, J., McDermott, G., Gros, M. A. L. & Larabell, C. A. Mesoscale imaging with cryo‐light and X‐rays: Larger than molecular machines, smaller than a cell. Biology of the Cell 109, 24–38 (2017).
  • Hammel, M., Amlanjyoti, D., Reyes, F. E., Chen, J.-H., Parpana, R., Tang, H. Y., Larabell, C. A., Tainer, J. A. & Adhya, S. HU multimerization shift controls nucleoid compaction. Science advances 2, e1600650 (2016).
  • Chen, J.-H., Kellner, Y., Zagrebelsky, M., Grunwald, M., Korte, M. & Walla, P. J. Two-photon correlation spectroscopy in single dendritic spines reveals fast actin filament reorganization during activity-dependent growth. PLoS One 10, e0128241 (2015). 
  • Hafi, N., Grunwald, M., Heuvel, L. S. V. D., Aspelmeier, T., Chen, J.-H., Zagrebelsky, M., Schütte, O. M., Steinem, C., Korte, M. & Munk, A. Fluorescence nanoscopy by polarization modulation and polarization angle narrowing. Nature methods 11, 579–584 (2014).