2020同步年報

Facility Status 099 Port No. Phase-III Beamline 2021 2022 2023 2024 2025 2026 TPS 11A In-situ Serial Protein Crystallography Beamline TPS 14A Small-angle X-ray Scattering Beamline TPS 20A Two Dimensional X-ray Di raction Beamline TPS 32A Tender X-ray Absorption Spectroscopy Beamline TPS 33A Dragon Beamline TPS 35A Soft X-ray Absorption Spectroscopy Beamline TPS 38A X-ray Absorption Spectroscopy Beamline TPS 43A TPS 47A High-resolution X-ray Absorption Spectroscopy Beamline Ambient Pressure X-ray Photoelectron Spectroscopy Beamline TPS 11A In-situ Serial Protein Crystallography Beamline The study of structure-function relations of macromolecules is a major aim in modern life science; synchrotron-based PX has become a powerful and fundamental technique to achieve that purpose. However, crystals of important mac- romolecules, such as membrane proteins, pathogenesis- related proteins and viruses, have typically small sizes and poor diffraction quality. In addition, some protein crystals are tougher to treat ( e.g . difficult to harvest or to freeze); these samples are so-called “hard-to-handle crystals”. Under this condition, NSRRC will build the third new PX beamline, TPS 11A , to tackle this challenging issue. The X-ray source of TPS 11A is an undulator (IU22) in vacuum and of length 3 m, producing a highly brilliant X-ray beam. TPS 11A will house a liquid-nitrogen-cooled horizontal double-crystal Fig. 1 : Construction schedule of TPS phase-III beamlines. Fig. 2 : TPS phase beamline map; phase I is shown in red, phase II in blue, and phase III in green. monochromator (DCM), a liquid-nitrogen-cooled double- multilayer monochromator (DMM), a horizontal focusing mirror (HFM) and a pair of Kirkpatrick-Baez mirrors to focus the beam to a fine spot. Among them, the DCM provides a high-energy resolution mode for phasing experiments de novo and the DMM offers usage mode with high pho- ton flux for high-throughput screening or data collection. The expected focused beam size at the sample position is designed for a range from 10 to 1 µ m. TPS 11A will be equipped with a single-photon-counting pixel detector and a high-speed robotic sample changer for automatic sample mounting and centering, making the data acquisition more efficient. TPS 11A , in particular, will provide four operating modes for PX communities: a standard goniometer, in-situ or tray (SBS plate) screening, fixed-target (microfluidic devices and thin-film sandwich) and injector (lipidic cubic