NSRRC Activity Report 2023

Life Science 047 This report features the work of Rey-Ting Guo and his collaborators published in Nature 621 , 840 (2023). TPS 05A Protein Microcrystallography TLS 15A1 Biopharmaceuticals Protein Crystallography • Protein Crystallography • Biological Macromolecules, Protein Structures, Life Science References 1. Y. Yang, L. Li, L. Yuan, X. Zhou, J. Duan, H. Xiao, N. Cai, S. Han, X. Ma, W. Liu, C.-C. Chen, L. Wang, X. Li, J. Chen, N. Kang, J. Chen, Z. Shen, S. R. Malwal, W. Liu, Y. Shi, E. Oldfield, R.-T. Guo, Y. Zhang, Immunity 50 , 1043 (2019). 2. M. Rigau, S. Ostrouska, T. S. Fulford, D. N. Johnson, K. Woods, Z. Ruan, H. E. G. McWilliam, C. Hudson, C. Tutuka, A. K. Wheatley, S. J. Kent, J. A. Villadangos, B. Pal, C. Kurts, J. Simmonds, M. Pelzing, A. D. Nash, A. Hammet, A. M. Verhagen, G. Vairo, E. Maraskovsky, C. Panousis, N. A. Gherardin, J. Cebon, D. I. Godfrey, A. Behren, A. P. Uldrich, Science 367 , eaay5516 (2020). 3. L. Yuan, X. Ma, Y. Yang, Y. Qu, X. Li, X. Zhu, W. Ma, J. Duan, J. Xue, H. Yang, J.-W. Huang, S. Yi, M. Zhang, N. Cai, L. Zhang, Q. Ding, K. Lai, C. Liu, L. Zhang, X. Liu, Y. Yao, S. Zhou, X. Li, P. Shen, Q. Chang, S. R. Malwal, Y. H, W. Li, C. Chen, C.-C. Chen, E. Oldfield, R.-T. Guo, Y. Zhang, Nature 621 , 840 (2023). Fig. 2 : Features of BTN2A1 B30.2 required for its association with BTN3A1 and for HMBPP-induced Vγ9Vδ2 T-cell activation. (a) Cytotoxicity of Vγ9Vδ2 T cells toward BTN2A−/− MIA PaCa-2 (2A1/2A2 KO, n=5, representative of two independent experiments) transfected with plasmids for the indicated 2A1 mutant variants pretreated with HMBPP (1 nM–100 µM) overnight. Note that the 2A1 ΔC mutant was BTN2A1 with a truncated tail (truncated residues include LTGANGVTPEEGLTLHRVSLLE). Error bars: SEM. (b) Flow cytometry analysis of His-tagged 2A1 mutant variants in BTN2A−/− 293T cells (n=4, representative of two independent experiments), as detected by anti-His mAb. Error bars: SEM. [Reproduced from Ref. 3]