NSRRC Activity Report 2023

046 NSRRC ACTIVITY REPORT 2023 Fig. 1 : The structures of the BTN2A1 B30.2 and BTN3A1 B30.2–HMBPP–BTN2A1 B30.2 complex. (a) Cartoon model of the apo BTN2A1 B30.2 crystal structure (left), electrostatic surface, and residues in the BTN2A1 B30.2 structure corresponding to BTN3A1 B30.2 (right). (b) The structure of the BTN3A1 B30.2–HMBPP–BTN2A1 B30.2 complex. Middle, cartoon representation of two BTN3A1 B30.2–HMBPP molecules (cyan and yellow) in complex with a BTN2A1 B30.2 homodimer (the A and B chains are shown in pink and green, respectively). Magnified views of the interactions between the BTN2A1 B30.2 dimer and the HMBPP–BTN3A1 B30.2 A chain are shown: HMBPP and the BTN2A1 and BTN3A1 B30.2 domains (i), BTN2A1 B30.2 and BTN3A1 B30.2 domains (ii, iii), and Trp350/Trp391 of BTN3A1 B30.2 and the BTN2A1 B30.2 domain (iv). Water molecules are shown as small red spheres. The black dashed lines indicate hydrogen bonds, and the purple dashed lines indicate salt bridges. [Reproduced from Ref. 3]