NSRRC Activity Report 2022

Life Science 055 cellular nuclei to execute their biological functions. 8,9 These case studies exemplify how SAXS provides key structural information on functional states of biologically important protein systems. The integration of size exclusion chromatography for online UV and SAXS detection, as has been implemented at TLS 23A1 and TPS 13A beamlines, has been instrumental in the success in obtaining high- quality SAXS data for difficult-to-analyze biological systems. (Reported by Shang-Te Danny Hsu, Academia Sinica) This report features the work of Shang-Te Danny Hsu and his collaborators published in J. Mol. Biol. 434 , 167553 (2022). TLS 23A1 Small/Wide Angle X-ray Scattering TPS 13A BioSAXS • Biological Small/Wide Angle X-ray Scattering • Biological Macromolecules, Protein Solution, Life Science References 1. M. H. Koch, P. Vachette, D. I. Svergun, Q. Rev. Biophys. 36 , 147 (2003). 2. Y. C. Chuang, I. C. Hu, P. C. Lyu, S.-T. D. Hsu, J. Mol. Biol. 431 , 857 (2019). 3. K. T. Ko, I. C. Hu, K. F. Huang, P. C. Lyu, S.-T. D. Hsu, Struc- ture 27 , 1224 (2019). 4. S.-T. D. Hsu, Y. C. Lee, K. M. Mikula, S. M. Backlund, I. Tas- con, A. Goldman, H. Iwai, Front. Chem. 9 , 663241 (2021). 5. Y. C. Lee, S.-T. D. Hsu, J. Phys. Chem. Lett. 9 , 2433 (2018). 6. I. Wang, S. Y. Chen, S.-T. D. Hsu, Sci. Rep. 6 , 31514 (2016). 7. I. Wang, S. Y. Chen, S.-T. D. Hsu, J. Phys. Chem. B 119 , 4359 (2015). 8. S. Puri, S. N. Chen, Y. H. Chiu, P. Draczkowski, K. T. Ko, T. J. Yang, Y. S. Wang, S. Uchiyama, S.-T. D. Hsu, J. Mol. Biol. 434 , 167553 (2022). 9. T. J. Yang, T. N. Li, R. S. Huang, M. Y. Pan, S. Y. Lin, S. Lin, K. P. Wu, L. H. Wang, S.-T. D. Hsu, J. Cell Biol. 221 , e202201094 (2022).