NSRRC Activity Report 2022

Appendix 149 Lim, and H. S. Yoon*( 尹浩燮 ), “ Prostaglandin A2 Interacts with Nurr1 and Ameliorates Behavioral Defcits in Parkinson’s Disease Fly Model ”, Neuromol. Med. 24 , 469 (2022). • R. Rattinam, R. S. Basha, Y.-L. Wang, Z.-C. Wang, N.-S. Hsu, K.-H. Lin, S. M. Zadeh, K. Adhikari, J.-P. Lin, and T.-L. Li*( 李宗璘 ), “ KasQ an Epimerase Primes the Biosynthesis of Aminoglycoside Antibiotic Kasugamycin and KasF/H Acetyltransferases Inactivate Its Activity ”, Biomedicines 10 , 212 (2022). • R. Satange, A. B. Rode, and M.-H. Hou*( 侯明宏 ), “ Revisiting Recent Unusual Drug-DNA Complex Structures: Implications for Cancer and Neurological Disease Diagnostics and Therapeutics ”, Bioorg. Med. Chem. 76 , 117094 (2022). • S. Shankar, Z.-T. Hsu, A. Ezquerra, C.-C. Li, T.-L. Huang, E. Coyaud, R. Viais, C. Grauffel, B. Raught, C. Lim, J. Luders*, S.-Y. Tsai*( 蔡素宜 ), and K.-C. Hsia* ( 夏國強 ), “ A γ-tubulin Complex-dependent Pathway Suppresses Ciliogenesis by Promoting Cilia Disassembly ”, Cell Rep. 41 , 111642 (2022). • D. Tanramluk*, D. Pakotiprapha, S. Phoochaijaroen, P. Chantravisut, S. Thampradid, J. Vanichtanankul, L. Narupiyakul, R. Akavipat, and J. Yuvaniyama, “ MANORAA: A Machine Learning Platform to Guide Protein-ligand Design by Anchors and Influential Distances ”, Structure 30 , 181 (2022). • N. Thamwiriyasati, C. Kanchanawarin, C. Imtong, C.-J. Chen, H.-C. Li, and C. Angsuthanasombat*, “ Complete Structure Elucidation of a Functional form of the Bacillus Thuringiensis Cry4Ba δ-endotoxin: Insights into Toxin-induced Transmembrane Pore Architecture ”, Biochem. Biophy. Res. Co. 620 , 158 (2022). • N. Tien, C.-Y. Ho, S.-J. Lai, Y.-C. Lin, C.-S. Yang, Y.-C. Wang, W.-C. Huang* ( 黃偉謙 ), Y. Chen*( 陳曄 ), and J.-J. Chang*( 張瑞仁 ), “ Crystal Structure of the Capsular Polysaccharide-synthesis Enzyme CapG from Staphylococcus Aureus ”, Acta Crystallogr. F 78 , 378 (2022). ◎ I.-F. Tu, T.-L. Lin, F.-L. Yang, I.-M. Lee, W.-L. Tu, J.-H. Liao, T.-P. Ko, W.-J. Wu, J.-T. Jan, M.-R. Ho, C.-Y. Chou, A. H.-J. Wang, C.-Y. Wu, J.-T. Wang, K.-F. Huang* ( 黃開發 ), and S.-H. Wu*( 吳世雄 ), “ Structural and Biological Insights into Klebsiella Pneumoniae Surface Polysaccharide Degradation by a Bacteriophage K1 Lyase: Implications for Clinical Use ”, J. Biomed. Sci. 29 , 9 (2022). • J. Vanichtanankul*, A. Yoomuang, S. Taweechai, T. Saeyang, J. Pengon, J. Yuvaniyama, B. Tarnchompoo, Y. Yuthavong, and S. Kamchonwongpaisan*, “ Structural Insight into Effective Inhibitors’ Binding to Toxoplasma Gondii Dihydrofolate Reductase Thymidylate Synthase ”, ACS Chem. Biol. 17 , 1691 (2022). • T.-J. Ye, K.-F. Huang, T.-P. Ko*( 柯子平 ), and S.-H. Wu*( 吳世雄 ), “ Synergic Action of an Inserted Carbohydrate-binding Module in a Glycoside Hydrolase Family 5 Endoglucanase ”, Acta Crystallogr. D 78 , 633 (2022). • S. M. Zadeh, M.-H. Chen, Z.-C. Wang, E. K. Astani, I.-W. Lo, K.-H. Lin, N.-S. Hsu, K. Adhikari, S.-Y. Lyu, H.-Y. Tsai, Y. Terasawa, M. Yabe, K. Yamamoto, S. Ichikawa, and T.-L. Li*( 李宗璘 ), “ β-hydroxylation of α-amino-β- hydroxylbutanoyl-glycyluridine Catalyzed by a Nonheme Hydroxylase Ensures the Maturation of Caprazamycin ”, Commun. Chem. 5 , 87 (2022). • Y.-Z. Zheng, J. Cui, Y.-L. Wang, S.-J. Huang, E.-C. Lin, S.-C. Huang, J. D. Rudolf, X. Yan, and C.-Y. Chang*( 張晉源 ), “ The Structure-function Relationship of Human Bleomycin Hydrolase: Mutation of a Cysteine Protease into a Serine Protease ”, ChemBioChem 23 , e202200186 (2022). TLS 13C1 SW60 − Protein Crystallography • P.-C. Chen, M.-H. Hsieh, W.-S. Kuo, L. S.-H. Wu, H.-F. Kao, L.-F. Liu, Z.-G. Liu, W.- Y. Jeng*( 鄭文義 ), and J.-Y. Wang*( 王志堯 ), “ Moonlighting Glyceraldehyde- 3-phosphate Dehydrogenase (GAPDH) Protein of Lactobacillus Gasseri Attenuates Allergic Asthma via Immunometabolic Change in Macrophages ”, J. Biomed. Sci. 29 , 75 (2022). • E.-S. Lin, R.-H. Luo, Y.-C. Yang, and C.-Y. Huang*( 黃晟洋 ), “ Molecular Insights into How the Dimetal Center in Dihydropyrimidinase Can Bind the Thymine Antagonist 5-aminouracil: A Different Binding Mode from the Anticancer Drug 5-fluorouracil ”, Bioinorg. Chem. Appl. 2022 , 1817745 (2022). • E.-S. Lin, R.-H. Luo, and C.-Y. Huang*( 黃晟洋 ), “ A Complexed Crystal Structure of a Single-stranded DNA-binding Protein with Quercetin and the Structural Basis of Flavonol Inhibition Specificity ”, Int. J. Mol. Sci. 23 , 588 (2022). • E.-S. Lin, Y.-H. Huang, R.-H. Luo, Z. Basharat, and C.-Y. Huang*( 黃晟洋 ), “ Crystal Structure of an SSB Protein from Salmonella Enterica and Its Inhibition by Flavanonol Taxifolin ”, Int. J. Mol. Sci. 23 , 4399 (2022). • S. Shankar, Z.-T. Hsu, A. Ezquerra, C.-C. Li, T.-L. Huang, E. Coyaud, R. Viais, C. Grauffel, B. Raught, C. Lim, J. Luders*, S.-Y. Tsai*( 蔡素宜 ), and K.-C. Hsia* ( 夏國強 ), “ A γ-tubulin Complex-dependent Pathway Suppresses Ciliogenesis by Promoting Cilia Disassembly ”, Cell Rep. 41 , 111642 (2022). ◎ I.-F. Tu, T.-L. Lin, F.-L. Yang, I.-M. Lee, W.-L. Tu, J.-H. Liao, T.-P. Ko, W.-J. Wu, J.-T. Jan, M.-R. Ho, C.-Y. Chou, A. H.-J. Wang, C.-Y. Wu, J.-T. Wang, K.-F. Huang* ( 黃開發 ), and S.-H. Wu*( 吳世雄 ), “ Structural and Biological Insights into Klebsiella Pneumoniae Surface Polysaccharide Degradation by a Bacteriophage K1 Lyase: Implications for Clinical Use ”, J. Biomed. Sci. 29 , 9 (2022). • S. M. Zadeh, M.-H. Chen, Z.-C. Wang, E. K. Astani, I.-W. Lo, K.-H. Lin, N.-S. Hsu, K. Adhikari, S.-Y. Lyu, H.-Y. Tsai, Y. Terasawa, M. Yabe, K. Yamamoto, S. Ichikawa, and T.-L. Li*( 李宗璘 ), “ β-hydroxylation of α-amino-β- hydroxylbutanoyl-glycyluridine Catalyzed by a Nonheme Hydroxylase Ensures the Maturation of Caprazamycin ”, Commun. Chem. 5 , 87 (2022). TLS 14A1 BM − IR Microscopy • A. A. Ayele, M.-C. Tsai*( 蔡孟哲 ), D. B. Adam, Y. A. Awoke, W.-H. Huang, C.-Y. Chang, S.-C. Liao, P.-Y. Huang, J.-L. Chen, C.-W. Pao, W.-N. Su*( 蘇威年 ), and B. J. Hwang*( 黃炳照 ), “ Electrochemical Oxidation of Ethylene Glycol on TiO 2 -supported Platinum Single-atom Catalyst into Valuable Chemicals in Alkaline Media ”, Appl. Catal. A-Gen. 646 , 118861 (2022). • P. P. Biswas, G. Turner-Walker, J. Rathod, B. Liang*( 梁碧清 ), C.-C. Wang, Y.-C. Lee, and H.-S. Sheu, “ Sustainable Phosphorus Management in Soil Using Bone Apatite ”, J. Environ. Manage. 305 , 114344 (2022). • Y.-T. Chen, P.-Y. Huang, J.-Y. Wang, Y.-C. Lee*( 李耀昌 ), and C.-Y. Chai*( 蔡志仁 ), “ An Innovative Diagnosis in Gastrointestinal Neuroendocrine Neoplasms Using Wax-physisorption-kinetics-based FTIR Imaging ”, Sci. Rep. 12 , 17168 (2022). • K. Lakshmanan, W.-H. Huang, S. A. Chala, B. W. Taklu, E. A. Moges, J.-F. Lee, P.-Y. Huang, Y.-C. Lee, M.-C. Tsai*( 蔡孟哲 ), W.-N. Su*( 蘇威年 ), and B. J. Hwang*( 黃炳照 ), “ Highly Active Oxygen Coordinated Configuration of Fe Single-atom Catalyst toward Electrochemical Reduction of CO 2 into Multi-carbon Products ”, Adv. Funct. Mater. 32 , 2109310 (2022). • P.-Y. Lin, P.-Y. Huang, Y.-C. Lee*( 李耀昌 ), and C. S. Ng*( 黃貞祥 ), “ Analysis and Comparison of Protein Secondary Structures in the Rachis of Avian Flight Feathers ”, PeerJ 10 , e12919 (2022). TLS 15A1 Biopharmaceuticals Protein Crystallography • H.-Y. Chang*( 張欣暘 ), L.-H. Lo, Y.-H. Lan, M.-X. Hong, Y. T. Chan, T.-P. Ko, Y.-R. Huang, T.-H. Cheng, and C.-C. Liaw*( 廖志中 ), “ Structural Insights into the Substrate Selectivity of α-oxoamine Synthases from Marine Vibrio sp. QWI-06 ”, Colloid. Surface. B 210 , 112224 (2022). • M.-H. Chen, Y.-S. Li, N.-S. Hsu, K.-H. Lin, Y.-L. Wang, Z.-C. Wang, C.-F. Chang, J.-P. Lin, C.-Y. Chang, and T.-L. Li*( 李宗璘 ), “ Structural and Mechanistic Bases for StnK3 and Its Mutant-mediated Lewis-acid-dependent Epimerization and Retro-aldol Reactions ”, ACS Catalysis 12 , 1945 (2022). • T. Chen, C. Grauffel, W.-Z. Yang, Y.-P. Chen, H. S. Yuan*( 袁小琀 ), and C. Lim*( 林小喬 ), “ Efficient Strategy to Design Protease Inhibitors: Application to Enterovirus 71 2A Protease ”, ACS Bio Med Chem Au 2 , 437 (2022). • Y.-H. Hsiao, S.-J. Huang, E.-C. Lin, P.-Y. Hsiao, S.-I. Toh, I.-H. Chen, Z. Xu, Y.-P. Lin, H.-J. Liu, and C.-Y. Chang*( 張晉源 ), “ Crystal Structure of the α-ketoglutarate-dependent Non-heme Iron Oxygenase CmnC in Capreomycin Biosynthesis and Its Engineering to Catalyze Hydroxylation of the Substrate Enantiomer ”, Front. Chem. 10 , 1001311 (2022). • J.-N. Hsu, J.-S. Chen, S.-M. Lin, J.-Y. Hong, Y.-J. Chen, U.-S. Jeng, S.-Y. Luo* ( 羅順原 ), and M.-H. Hou*( 侯明宏 ), “ Targeting the N-terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation ”, Front. Mol. Biosci. 9 , 871499 (2022). ◎ H.-Y. Huang, H.-Y. Liao, X. Chen, S.-W. Wang, C.-W. Cheng, M. Shahed-Al- Mahmud, Y.-M. Liu, A. Mohapatra, T.-H. Chen, J. M. Lo, Y.-M. Wu, H.-H. Ma, Y.- H. Chang, H.-Y. Tsai, Y.-C. Chou, Y.-P. Hsueh, C.-Y. Tsai, P.-Y. Huang, S.-Y. Chang, T.-L. Chao, H.-C. Kao, Y.-M. Tsai, Y.-H. Chen, C.-Y. Wu, J.-T. Jan, T.-J. R. Cheng, K.-I. Lin*( 林國儀 ), C. Ma*( 馬徹 ), and C.-H. Wong*( 翁啟惠 ), “ Vaccination with SARS-CoV-2 Spike Protein Lacking Glycan Shields Elicits Enhanced Protective Responses in Animal Models ”, Sci. Transl. Med. 14 , eabm0899 (2022). • M.-S. Lee, K.-Y. Hsieh, C.-I. Kuo, S.-H. Lee, and C.-I. Chang*( 張崇毅 ), “ Structural Basis for the Peptidoglycan-editing Activity of YfiH ”, mBio 13 , e03646-21 (2022). • C.-C. Lin, S. Y. Hoo, L.-T. Ma, C. Lin, K.-F. Huang, Y.-N. Ho, C.-H. Sun, H.-J. Lee, P.-Y. Chen, L.-J. Shu, B.-W. Wang, W.-C. Hsu, T.-P. Ko, and Y.-L. Yang*( 楊玉良 ), “ Integrated Omics Approach to Unveil Antifungal Bacterial Polyynes as Acetyl-CoA Acetyltransferase Inhibitors ”, Commun. Biol. 5 , 454 (2022). • S. R. Malwal, N. Shang, W. Liu, X. Li, L. Zhang, C.-C. Chen, R.-T. Guo, and E. Oldfield*, “ A Structural and Bioinformatics Investigation of a Fungal Squalene Synthase and Comparisons with Other Membrane Proteins ”, ACS Omega 7 , 22601 (2022). • P.-J. Pao, M.-F. Hsu, M.-H. Chiang, C.-T. Chen, C.-C. Lee*, and A. H.-J. Wang* ( 王惠鈞 ), “ Structural Basis of an Epitope Tagging System Derived from Haloarcula Marismortui Bacteriorhodopsin I D94N and Its Monoclonal Antibody GD-26 ”, FEBS J. 289 , 730 (2022). • R. Rattinam, R. S. Basha, Y.-L. Wang, Z.-C. Wang, N.-S. Hsu, K.-H. Lin, S. M. Zadeh, K. Adhikari, J.-P. Lin, and T.-L. Li*( 李宗璘 ), “ KasQ an Epimerase Primes the Biosynthesis of Aminoglycoside Antibiotic Kasugamycin and KasF/H