NSRRC Activity Report 2022

134 NSRRC ACTIVITY REPORT 2022 Note: 1. Asterisks (*) indicate corresponding authors. Their Chinese names are shown in parentheses if applicable. 2. Publications are sorted by endstation and listed in alphabetic order according to the last name of the first author. 3. Publications using more than one beamline are listed in corresponding beamline sections. 4. Publications marked ◎ are selected to report in the section of Research Highlights. TPS 05A Protein Microcrystallography • C. Akıl, S. Ali, L. T. Tran, J. Gaillard, W. Li, K. Hayashida, M. Hirose, T. Kato, A. Oshima, K. Fujishima, L. Blanchoin, A. Narita*, and R. C. Robinson*, “ Structure and Dynamics of Odinarchaeota Tubulin and the Implications for Eukaryotic Microtubule Evolution ”, Sci. Adv. 8 , eabm2225 (2022). • C. Akıl, L. T. Tran, M. Orhant-Prioux, Y. Baskaran, Y. Senju, S. Takeda, P. Chotchuang, D. Muengsaen, A. Schulte, E. Manser, L. Blanchoin, and R. C. Robinson*, “ Structural and Biochemical Evidence for the Emergence of a Calcium- regulated Actin Cytoskeleton Prior to Eukaryogenesis ”, Commun. Biol. 5 , 890 (2022). • Y. An, Z. S. Chen, H. Y. E. Chan*( 陳浩然 ), and J. C. K. Ngo*( 敖志祺 ), “ Molecular Insights into the Interaction of CAG Trinucleotide RNA Repeats with Nucleolin and Its Implication in Polyglutamine Diseases ”, Nucleic Acids Res. 50 , 7655 (2022). • J. Anantharajan, N. Baburajendran, G. Lin, Y. Y. Loh, W. Xu, N. H. B. Ahmad, S. Liu, A. E. Jansson, J. W. L. Kuan, E. Y. Ng, Y. K. Yeo, A. W. Hung, J. Joy*, J. Hill, H. L. Ford, R. Zhao, T. H. Keller*, and C. B. Kang*, “ Structure-activity Relationship Studies of Allosteric Inhibitors of EYA2 Tyrosine Phosphatase ”, Protein Sci. 31 , 422 (2022). • C.-C. Chen, M. Dai, L. Zhang, J. Zhao, W. Zeng, M. Shi, J.-W. Huang, W. Liu, R.-T. Guo*( 郭瑞庭 ), and A. Li*( 李愛濤 ), “ Molecular Basis for a Toluene Monooxygenase to Govern Substrate Selectivity ”, ACS Catalysis 12 , 2831 (2022). • I.-H. Chen, T. Cheng, Y.-L. Wang, S.-J. Huang, Y.-H. Hsiao, Y.-T. Lai, S.-I. Toh, J. Chu, J. D. Rudolf, and C.-Y. Chang*( 張晉源 ), “ Characterization and Structural Determination of CmnG-A, the Adenylation Domain that Activates the Nonproteinogenic Amino Acid Capreomycidine in Capreomycin Biosynthesis ”, ChemBioChem 23 , e202200563 (2022). • M.-H. Chen, Y.-S. Li, N.-S. Hsu, K.-H. Lin, Y.-L. Wang, Z.-C. Wang, C.-F. Chang, J.-P. Lin, C.-Y. Chang, and T.-L. Li*( 李宗璘 ), “ Structural and Mechanistic Bases for StnK3 and Its Mutant-mediated Lewis-acid-dependent Epimerization and Retro-aldol Reactions ”, ACS Catalysis 12 , 1945 (2022). • T. Chen, C. Grauffel, W.-Z. Yang, Y.-P. Chen, H. S. Yuan*( 袁小琀 ), and C. Lim*( 林小喬 ), “ Efficient Strategy to Design Protease Inhibitors: Application to Enterovirus 71 2A Protease ”, ACS Bio Med Chem Au 2 , 437 (2022). • C.-Y. Ho, J.-X. Yu, Y.-C. Wang, Y.-C. Lin, Y.-F. Chiu, J.-Y. Gao, S.-J. Lai, M.-J. Chen, W.-C. Huang, N. Tien*( 田霓 ), and Y. Chen*( 陳曄 ), “ A Structural Comparison of SARS-CoV-2 Main Protease and Animal Coronaviral Main Protease Reveals Species- specific Ligand Binding and Dimerization Mechanism ”, Int. J. Mol. Sci. 23 , 5669 (2022). • Y.-H. Hsiao, S.-J. Huang, E.-C. Lin, P.-Y. Hsiao, S.-I. Toh, I.-H. Chen, Z. Xu, Y.-P. Lin, H.-J. Liu, and C.-Y. Chang*( 張晉源 ), “ Crystal Structure of the α-ketoglutarate-dependent Non-heme Iron Oxygenase CmnC in Capreomycin Biosynthesis and Its Engineering to Catalyze Hydroxylation of the Substrate Enantiomer ”, Front. Chem. 10 , 1001311 (2022). • J.-N. Hsu, J.-S. Chen, S.-M. Lin, J.-Y. Hong, Y.-J. Chen, U.-S. Jeng, S.-Y. Luo* ( 羅順原 ), and M.-H. Hou*( 侯明宏 ), “ Targeting the N-terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation ”, Front. Mol. Biosci. 9 , 871499 (2022). • M.-J. C. Y. Kee, S. R. Bharath, S. Wee, M. W. Bowler, J. Gunaratne, S. Pan, L. List of Publications Publications (including conference proceedings) listed here are based on the results obtained from facilities associated with NSRRC. Zhang, and H. Song*( 宋海威 ), “ Structural Insights into the Substrate-bound Condensation Domains of Non-ribosomal Peptide Synthetase AmbB ”, Sci. Rep. 12 , 5353 (2022). ◎ T.-P. Ko, Y.-C. Wang, C.-S. Yang, M.-H. Hou, C.-J. Chen, Y.-F. Chiu, and Y. Chen* ( 陳曄 ), “ Crystal Structure and Functional Implication of Bacterial STING ”, Nat. Commun. 13 , 26 (2022). • J. V. Kumar, T.-S. Tseng, Y.-C. Lou, S.-Y. Wei, T.-H. Wu, H.-C. Tang, Y.-C. Chiu, C.- H. Hsu*( 徐駿森 ), and C. Chen*( 陳金榜 ), “ Structural Insights into DNA Binding Domain of Vancomycin-resistance-associated Response Regulator in Complex with Its Promoter DNA from Staphylococcus Aureus ”, Protein Sci. 31 , e4286 (2022). • W.-H. Lee, X. Chen, I.-J. Liu, J.-H. Lee, C.-M. Hu, H.-C. Wu, S.-K. Wang, W.-H. Lee*( 李文華 ), and C. Ma*( 馬徹 ), “ Structural Basis of Interleukin-17B Receptor in Complex with a Neutralizing Antibody for Guiding Humanization and Affinity Maturation ”, Cell Rep. 41 , 111555 (2022). ◎ H. Li, Y. Yang, Y. Hu, C.-C. Chen, J.-W. Huang, J. Min, L. Dai*( 戴隆海 ), and R.-T. Guo*( 郭瑞庭 ), “ Structural Analysis and Engineering of Aldo-keto Reductase from Glyphosate-resistant Echinochloa Colona ”, J. Hazard. Mater. 436 , 129191 (2022). • C.-C. Lin, S. Y. Hoo, L.-T. Ma, C. Lin, K.-F. Huang, Y.-N. Ho, C.-H. Sun, H.-J. Lee, P.-Y. Chen, L.-J. Shu, B.-W. Wang, W.-C. Hsu, T.-P. Ko, and Y.-L. Yang*( 楊玉 良 ), “ Integrated Omics Approach to Unveil Antifungal Bacterial Polyynes as Acetyl- CoA Acetyltransferase Inhibitors ”, Commun. Biol. 5 , 454 (2022). • M. Maestre-Reyna, C.-H. Yang, E. Nango, W.-C. Huang, E. P. G. N. Putu, W.-J. Wu, P.-H. Wang, S. Franz-Badur, M. Saft, H. Emmerich, H.-Y. Wu, C.-C. Lee, K.-F. Huang, Y.-K. Chang, J.-H. Liao, J.-H. Weng, W. Gad, C.-W. Chang, A. H. Pang, M. Sugahara, S. Owada, Y. Hosokawa, Y. Joti, A. Yamashita, R. Tanaka, T. Tanaka, F. Luo, K. Tono, K.-C. Hsu, S. Kiontke, I. Schapiro, R. Spadaccini, A. Royant, J. Yamamoto, S. Iwata, L. Essen*, Y. Bessho*, and M.-D. Tsai*( 蔡明道 ), “ Serial Crystallography Captures Dynamic Control of Sequential Electron and Proton Transfer Events in a Flavoenzyme ”, Nat. Chem. 14 , 677 (2022). • R. Rattinam, R. S. Basha, Y.-L. Wang, Z.-C. Wang, N.-S. Hsu, K.-H. Lin, S. M. Zadeh, K. Adhikari, J.-P. Lin, and T.-L. Li*( 李宗璘 ), “ KasQ an Epimerase Primes the Biosynthesis of Aminoglycoside Antibiotic Kasugamycin and KasF/H Acetyltransferases Inactivate Its Activity ”, Biomedicines 10 , 212 (2022). • R. Satange, S.-H. Kao, C.-M. Chien, S.-H. Chou, C.-C. Lin, S. Neidle*, and M.-H. Hou*( 侯明宏 ), “ Staggered Intercalation of DNA Duplexes with Base- pair Modulation by Two Distinct Drug Molecules Induces Asymmetric Backbone Twisting and Structure Polymorphism ”, Nucleic Acids Res. 50 , 8867 (2022). • R. Satange, A. B. Rode, and M.-H. Hou*( 侯明宏 ), “ Revisiting Recent Unusual Drug-DNA Complex Structures: Implications for Cancer and Neurological Disease Diagnostics and Therapeutics ”, Bioorg. Med. Chem. 76 , 117094 (2022). • S. Shankar, Z.-T. Hsu, A. Ezquerra, C.-C. Li, T.-L. Huang, E. Coyaud, R. Viais, C. Grauffel, B. Raught, C. Lim, J. Luders*, S.-Y. Tsai*( 蔡素宜 ), and K.-C. Hsia* ( 夏國強 ), “ A γ-tubulin Complex-dependent Pathway Suppresses Ciliogenesis by Promoting Cilia Disassembly ”, Cell Rep. 41 , 111642 (2022). • Y. B. Tan, L. S. Lello, X. Liu, Y.-S. Law, C. Kang, J. Lescar, J. Zheng, A. Merits*, and D. Luo*( 羅大海 ), “ Crystal Structures of Alphavirus Nonstructural Protein 4(nsP4) Reveal an Intrinsically Dynamic RNA-dependent RNA Polymerase Fold ”, Nucleic Acids Res. 50 , 1000 (2022). • T. Wang, Y. Yang, M. He, M. Liu, J.-W. Huang, J. Min, C.-C. Chen, Y. Liu* ( 劉映樂 ), L. Zhang*( 張麗蘭 ), and R.-T. Guo*( 郭瑞庭 ), “ Structural Insights into the Cyclization of Unusual Brasilane-type Sesquiterpenes ”, Int. J. Biol. Macromol. 209 , 1784 (2022). • T.-J. Yang, T.-N. Li, R.-S. Huang, M. Y.-C. Pan, S.-Y. Lin, S. Lin, K.-P. Wu, L. H.-C. Wang, and S.-T. D. Hsu*( 徐尚德 ), “ Tumor Suppressor BAP1 Nuclear Import is Governed by Transportin-1 ”, J. Cell Biol. 221 , e202201094 (2022). • T.-J. Ye, K.-F. Huang, T.-P. Ko*( 柯子平 ), and S.-H. Wu*( 吳世雄 ), “ Synergic Action of an Inserted Carbohydrate-binding Module in a Glycoside Hydrolase Family 5 Endoglucanase ”, Acta Crystallogr. D 78 , 633 (2022). • S. M. Zadeh, M.-H. Chen, Z.-C. Wang, E. K. Astani, I.-W. Lo, K.-H. Lin, N.-S. Hsu, K. Adhikari, S.-Y. Lyu, H.-Y. Tsai, Y. Terasawa, M. Yabe, K. Yamamoto, S. Ichikawa, and T.-L. Li*( 李宗璘 ), “ β-hydroxylation of α-amino-β- hydroxylbutanoyl-glycyluridine Catalyzed by a Nonheme Hydroxylase Ensures the