0428同步年報-2021-全

040 ACTIVITY REPORT 2021 Life Science A dvanced methods of structural biology have accelerated modern research in life science and promoted many biomedical applications. Among these methods, synchrotron protein crystallography remains the incomparable tool to determine the structures of important biomolecules in apo and ligand-bound forms on an atomic scale to elucidate the mechanisms of complicated biological processes and to improve the development of drug design. In addition to TLS 15A1 and TPS 05A , a new beamline, TPS 07A , for micro-focus protein crystallography has been open to users since the beamtime of the 2021-2 cycle; this beamline is particularly optimized for micro- crystals and large inhomogeneous crystals. For other experimental purposes, five beamlines, including small- angle X-ray scattering ( TPS 13A ), soft X-ray tomography ( TPS 24A ), transmission X-ray microscopy ( TPS 31A ), quick- scanning X-ray absorption spectroscopy ( TPS 44A ) and infrared microspectroscopy ( TLS 14A1 ), can be selected to analyze bio-samples to unravel the unsolved problems in biology. The following outstanding studies were performed by our user communities of life science in 2021. Six reports include a plastic-eating enzyme, PETase, that can be applied to diminish plastic pollution by Rey-Ting Guo; a recognition mode deduced from the complex structure of G4C2 DNA with metal II -(Chro) 2 for drug design to treat effectively ALS and FTD neurological diseases by Ming-Hon Hou; various bacterial cyclic dinucleotides and trinucleotide synthases involved in defense against viral infections by Yeh Chen; the biradical mechanism of PQQ-dependent hydride transfer for the development of a biomimetic catalyst by Chun-Jung Chen; the anticancer drug-resistant mechanism associated with APE1 for strategic drug design by Yu-Yuan Hsiao; a Poa1p macro domain, the first enzyme possessing a non- canonical 3”- O AADPR deacetylase activity by Chun-Hua Hsu. (by Chun-Hsiang Huang)