Structural Insight into Catalytic Mechanism of PET Hydrolase
X. Han, W. Liu, J.-W. Huang, J. Ma, Y. Zheng, T.-P. Ko, L. Xu, Y.-S. Cheng, C.-C. Chen, and R.-T. Guo*
2018/02/01
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.
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